• Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

  • Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as: Have you selected the most appropriate research strategy? Have you identified the issues critical to your successful application of a technique? Are you familiar with the limitations of a given technique? When should common procedural rules of thumb not be applied? What strategies could you apply to resolve a problem? A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include: Working safely with biological samples and radioactive materials DNA and RNA purification PCR Protein and nucleid acid hybridization Prokaryotic and eukaryotic expression systems Properly using and maintaining laboratory equipment

  • PREFACE. CONTRIBUTORS. 1 INTRODUCTION AND OVERVIEW (David L. Kirchman). Eukaryotic Phytoplankton and Cyanobacteria. Photoheterotrophic Bacteria. Dissolved Organic Material. Heterotrophic Bacteria. Marine Archaea. Heterotrophic Protists. Nanoflagellates (2-20 µm). Microzooplanktonic Protists (20-200 µm). Dinoflagellates. Marine Fungi. Marine Viruses. N2 Fixers. Nitrifiers and Other Chemolithotrophs. Denitrifiers. 2 UNDERSTANDING ROLES OF MICROBES IN MARINE PELAGIC FOOD WEBS: A BRIEF HISTORY (Evelyn Sherr and Barry Sherr). Pre-1950s: The Early Years. 1950-1974. 1970s-1980s. Improvement in Methods. Bacterial Abundance. Bacterial Activity. Marine Heterotrophic Protists. The "Microbial Loop". 1990-Present: The Molecular Revolution. 3 BACTERIAL AND ARCHAEAL COMMUNITY STRUCTURE AND ITS PATTERNS (Jed A. Fuhrman and Ake Hagstrom). Major Groups of Prokaryotes in Seawater. "Classically" Culturable Bacteria. The Roseobacter Clade of Marine Alphaproteobacteria. Gammaproteobacteria. Bacteroidetes. Cyanobacteria. "Sea Water" Culturable Bacteria. SAR11 Cluster. Not-Yet-Cultured Bacteria. Marine Gammaproteobacterial Clusters. Actinobacteria. SAR116 Cluster. SAR202. Marine Group A. Marine Group B. Betaproteobacteria. Marine Archaea. Bacterioplankton Diversity. Species Concept. Microdiversity. Components of Diversity: Richness and Evenness. Community Structure: Description and Factors. Bottom-Up Control. Sideways Control. Top-Down Control. "Kill the Winner" Hypothesis. Temporal Variation (Days to Seasonal). Short-Term Variation. Seasonal Variation. Spatial Variation. Microscale Patterns. Global Distribution. Latitudinal Gradient and Degree of Endemism. Patchiness and Large Eddies. 4 GENOMICS AND METAGENOMICS OF MARINE PROKARYOTES (Mary Ann Moran). The Basics of Prokaryotic Genomics. Genome Sequence and Assembly. Finding Genes. Finding Operons. Functional Annotation. Tame or Wild? Pure-Culture Genomics Versus Metagenomics. Genomics in Marine Microbial Ecology. The Ecology of Genome Composition. Reverse Biogeochemistry: Discovery of New Ecological Processes. Environmental Reductionism: New Details About Recognized Processes. Comparative Genomics and Metagenomics. Future Directions. 5 PHOTOHETEROTROPHIC MARINE PROKARYOTES (Oded Beja and Marcelino T. Suzuki). Facultative Photoheterotrophy by Unicellular Cyanobacteria. Cyanobacteria as Facultative Heterotrophs. Uptake of Urea and DMSP. Uptake of Nucleosides and Amino Acids. Field Studies Using Light and Dark Incubations. Implications of Facultative Photoheterotrophy by Cyanobacteria. Marine AAnP Bacteria: Habitats and Diversity. Rediscovery of the Marine AAnP Bacteria. Diversity of AAnP Bacteria. Physiology of AAnP Bacteria. AAnP Bacterial Abundance and Ecological Significance. Proteorhodopsin-Containing Prokaryotes. Proteorhodopsin Genotypes and Taxonomic Distributions. Proteorhodopsin Spectral Tuning. Proteorhodopsin-Containing Prokaryotes: Abundance and Activity. Proteorhodopsin-Containing Prokaryotes: Ecological Significance. 6 ECOLOGY AND DIVERSITY OF PICOEUKARYOTES (Alexandra Z. Worden and Fabrice Not). Functional Roles, Classification, and Biological Traits. Photoautotrophs. Heterotrophs and Alternative Lifestyles. Environmental Diversity and Molecular Phylogenetics. Diversity of Uncultured Populations. Methodological Issues for envPCR Studies. Distribution, Abundance, and Activities. Methods for Quantifying Mixed Assemblages. Distribution, Abundance, and Activity of Mixed Picophytoplankton Assemblages. Quantifying Specific Picoeukaryote Populations. Methodological Challenges to Quantifying Specific Populations and Resolving Dynamics. Mortality, Contributions to Microbial Food Webs, and Microbial Interactions. Genomic Approaches to Picoeukaryote Ecology. Integration of Picoeukaryotes to the Microbial Food Web: Research Directions. 7 ORGANIC MATTER-BACTERIA INTERACTIONS IN SEAWATER (Toshi Nagata). Organic Matter Inventory and Fluxes. DOM-Bacteria Interactions. Labile Low-Molecular Weight (LMW) DOM. Extracellular Hydrolytic Enzymes. Polymeric DOM-Protein as a Model. Refractory DOM. POM-Bacteria Interactions. POM Continuum. POM Fluxes. POM-Mineral Interactions. Bacterial Community Structure and Utilization of Organic Matter. Future Challenges. 8 PHYSIOLOGICAL STRUCTURE AND SINGLE-CELL ACTIVITY IN MARINE BACTERIOPLANKTON (Paul A. del Giorgio and Josep M. Gasol). Distribution of Physiological States in Bacterioplankton Assemblages. The Concept of "Physiological Structure" of Bacterioplankton Assemblages. Starvation, Dormancy, and Viability in Marine Bacterioplankton. Describing the Physiological Structure of Bacterioplankton. Single-Cell Properties and Methodological Approaches. Operational Categories of Single-Cell Activity. Regulation of Physiological Structure of Marine Bacterioplankton. Factors Influencing Physiological State of Bacterial Cells in Marine Ecosystems. Factors Influencing Loss and Persistence of Physiological Fractions. Distribution of Single-Cell Characteristics in Marine Bacterioplankton Assemblages. Distribution of Single-Cell Activity and Physiological States in Marine Bacterioplankton. Simultaneous Determination of Several Aspects of Single-Cell Activity and Physiology. Patterns in Distribution of Single-Cell Activity and Physiology Along Marine Gradients. Distribution of Activity and Growth Among Bacterial Size Classes. Distribution of Activity Across and Within Major Phylogenetic Groups. Dynamics of Single-Cell Activity and Physiological States. Ecological Implications of Patterns in Bacterioplankton Single-Cell Activity. Community Versus Individual Cell Growth and Metabolic Rates. Linking the Distribution of Single-Cell Parameters and the Bulk Assemblage Response. Ecological Role of Different Physiological Fractions. 9 HETEROTROPHIC BACTERIAL RESPIRATION (Carol Robinson). Measurement of Bacterial Respiration and Production. Routine Measurement Techniques for Bacterial Respiration and Their Limitations. Routine Measurement Techniques for Bacterial Production and Their Limitations. Magnitude and Variability of Bacterial Respiration. Temporal Variability. Spatial Variability. Relationship Between Bacterial Respiration and Environmental and Ecological Factors. Bacterial Respiration as a Proportion of Community Respiration. Predicting Bacterial Respiration. Comparison Between Measurements and Predictions of Bacterial Respiration. Magnitude of Bacterial Respiration in Relation to Primary Production. Bacterial Respiration in a Changing Environment. 10 RESOURCE CONTROL OF BACTERIAL DYNAMICS IN THE SEA (Matthew J. Church). Growth in the Sea. Growth and Nutrient Uptake Kinetics. Approaches to Understanding Resource Control of Growth. Comparative Approaches. Experimental Approaches for Defining Limitation of Bacterial Growth. Limitation by Dissolved Organic Matter. Bacterial Growth on Bulk DOM Pools. Limitation by Specific DOM Compounds. Limitation by Inorganic Nutrients. Nitrogen. Phosphorus. Trace Nutrients. Temperature-DOM Interactions. Light. Resource Control of Specific Bacterial Populations in the Sea. 11 PROTISTAN GRAZING ON MARINE BACTERIOPLANKTON (Klaus Ju¨rgens and Ramon Massana). New Insights into Phylogenetic Organization. Functional Size Classes of Protists. Natural Assemblages of Marine Heterotrophic Nanoflagellates. Functional Ecology of Bacterivorous Flagellates. Living in a Dilute Environment. Using Culture Experiments to Infer the Ecological Role of HNF. Impact of Protistan Bacterivory on Marine Bacterioplankton. Search for the Perfect Method to Quantify Protistan Bacterivory. Rates of Protistan Bacterivory in the Sea. Balance of Bacterial Production and Protistan Grazing. Bottom-Up Versus Top-Down Control of Bacteria and Bacterivorous Protists. Ecological Functions of Bacterial Grazers. Grazing as a Shaping Force of Bacterial Assemblages. Bacterial Cell Size Determines Vulnerability Towards Grazers. Other Antipredator Traits of Prokaryotes. Grazing-Mediated Changes in Bacterial Community Composition. Molecular Tools for Protistan Ecology. Culturing Bias and Molecular Approaches. Global Distribution and Diversity of Marine Protists. Linking Diversity and Function for Uncultured Heterotrophic Flagellates. 12 MARINE VIRUSES: COMMUNITY DYNAMICS, DIVERSITY AND IMPACT ON MICROBIAL PROCESSES (Mya Breitbart, Mathias Middelboe, and Forest Rohwer). Viruses and the Marine Microbial Food Web. Direct Counts and Viral Numbers. Viral Production and Decay Rates. Viral Decay and Rates of Production in Pelagic Systems. Measurements of Viral Production in Marine Sediments. General Rates of Viral Production. Role of Viruses in Biogeochemical Cycling. Impact of Viruses on Bacterial Diversity and Community Dynamics. Marine Viral Diversity. Methods for Examining Marine Viral Diversity. Culture-Based Studies of Viral Diversity. The Need for Culture-Independent Methods. Culture-Independent Studies of Viral Diversity Using Transmission Electron Microscopy. Whole-Genome Profiling of Viral Communities Based on Genome Size. Studies of Viral Diversity Using Signature Genes. Metagenomic Studies of Viral Diversity. A Vision for the Future. 13 MOLECULAR ECOLOGICAL ASPECTS OF NITROGEN FIXATION IN THE MARINE ENVIRONMENT (Jonathan P. Zehr and Hans W. Paerl). Chemistry, Biochemistry, and Genetics of N2 Fixation. Genetics and Enzymology. Evolution of N2 Fixation. Phylogeny of Nitrogenase. Genomics of N2 Fixation. Diversity of N2-Fixing Microorganisms. Regulation in Diazotrophs. Methods for Assessing Diazotroph Diversity, Gene Expression, and N2 Fixation Activity. Ecophysiological Aspects of N2 Fixation. Ecology of Diazotrophs in the Open Ocean. Estuarine and Coastal Waters. Benthic Habitats, Including Microbial Mats and Reefs. Deep Water and Hydrothermal Vents. 14 NITROGEN CYCLING IN SEDIMENTS (Bo Thamdrup and Tage Dalsgaard). Inputs. Transformations. Microbes and Microbial Processes. Processes Involving Mn and Fe. Nitrogen Budgets. Benthic Budgets. Oceanic Budgets. Summary. References. INDEX.

  • Mitochondria

    Immo E. Scheffler

    "This volume inspires. It certainly will be much appreciated by cell biologists all over the world."
    Quarterly Review of Biology, March 2009 This book is the eagerly awaited second edition of the best-selling Mitochondria, a book widely acknowledged as the first modern, truly comprehensive authored work on the important, scientifically fundamental topic of the cellular organelles known as mitochondria. This new edition brings readers completely up to date on the many significant findings that have occurred in the eight years since the book was first published. As in that seminal first edition, the second edition tackles the biochemistry, genetics, and pathology of mitochondria in different organisms. The new edition provides thorough updates of all literature concerning this vital organelle, its functions, ongoing research surrounding it, and its importance vis-à-vis a broad range of issues in cellular and molecular biology. The book includes detailed descriptions of current and developing technologies around mitochondrial research and discovery, and highlights subjects that are growing, such as the use of proteomics. This book is an invaluable resource for all geneticists, biologists, and educators in life sciences. It is also of interest for advanced students in genetics and molecular biology.

  • The Practical Guide to The Genetic Family History Robin L. Bennett Compiling the most recent genetic developments in medical specialties, The Practical Guide to the Genetic Family History is a valuable resource which outlines the proper methods for taking and recording a patient's family medical history, allowing primary care physicians to be more efficient in diagnosing conditions with potential genetic components. With genetic screening forms, an overview of directed questions, pedigree nomenclature, and outlining common approaches used, genetic counselor Robin L. Bennett provides readers with the basic foundation in human genetics necessary to recognize inherited disorders and familial disease susceptibility in patients. As the only guide which is geared for the physician in this field, The Practical Guide to the Genetic Family History includes remarks by renowned medical geneticist Arno Motulsky, as well as information on structuring an accurate pedigree and its components, including:
    * Using a pedigree to identify individuals with an increased susceptibility to cancer
    * Family history, adoption, and their challenges
    * The connection between the pedigree and assisted reproductive technologies
    * Making referrals for genetic services
    * Neurological and neuromuscular conditions
    * Tables covering hearing loss, mental retardation, dementia, and seizures
    * Five case studies of genetics in practice
    An essential reference for genetics clinics, medical geneticists, and counselors, The Practical Guide to the Genetic Family History is also an invaluable aid for both primary care and specialist physicians who need an up-to-date reference that emphasizes both the science and art of modern clinical genetics.

  • The new edition of a classic reference incorporating the latest findings and discoveries
    The Third Edition of this classic reference provides readers with concise, up-to-the-moment coverage of the role of microorganisms in water and wastewater treatment. By providing a solid foundation in microbiology, microbial growth, metabolism, and nutrient cycling, the text gives readers the tools they need to make critical decisions that affect public health, as well as the practical aspects of treatment, disinfection, water distribution, bioremediation, and water and wastewater reuse.
    The publication begins a discussion of microbiology principles, followed by a discussion of public health issues and concerns. Next, the core of the text is dedicated to a thorough examination of wastewater and drinking water treatment, biosolids, pollution-control biotechnology, and drinking water distribution. The remainder of the text discusses toxicity testing in wastewater treatment plants, and the public health aspects of wastewater disposal and reuse.
    The many advances in wastewater and drinking water microbiology have all been thoroughly integrated into the publication, including:
    * A new chapter on bioterrorism and drinking water safety
    * The latest developments in biofilm microbial ecology and biofilm impact on drinking water quality
    * New, state-of-the-art detection techniques
    * Expanded and revised treatment of toxicity testing, including new testing methods and studies on endocrine disrupters in wastewater
    * Alternatives to conventional wastewater treatment
    New problem sets, which test readers' knowledge, as well as a list of Internet resources have been added to each chapter. In addition, the publication's extensive references have been thoroughly revised for readers who would like to learn more about the latest findings and discoveries on specialized topics. Finally, the color plate section has been expanded and contains many new illustrations and tables.
    An authoritative guide for all researchers, administrators, and engineers in the field of microbiology, Wastewater Microbiology, Third Edition is also a valuable reference for civil and environmental engineers, public health officials, and students involved in environmental engineering and science.

  • Tailoring retirement for successful business leaders  Traditional retirement planning fails to meet the needs of wealthy baby boomers, particularly those who are business leaders. There is no "one size fits all" answer.  Wealth Regeneration at Retirement: Planning for a Lifetime of Leadership presents an alternative - one that acts more like a GPS.   The authors, Kaycee Krysty and Bob Moser, leaders of the highly regarded Seattle-based wealth management firm, Laird Norton Tyee, use a proprietary discipline, Wealth Regeneration®, to calculate the route to retirement and beyond for those at the top. The authors challenge successful boomers to redefine retirement on their own terms. They outline a process to create a sustainable plan to achieve retirement objectives. Their years of experience in counseling CEO's and business founders through transitions is reflected throughout.  For many successful boomers, the answer to the prospect of retirement has been, "I'd rather not." Yet change is inevitable. Wealth Regeneration at Retirement provides a thoughtful and thorough way for leaders to move onward.  Describing Wealth Regeneration in a digestible, actionable format, the book provides the framework, tools, and techniques that successful baby boomers and their advisors need to incorporate this innovative approach for a lifetime of leadership and legacy. Packed with learning aids, including graphics, diagrams, worksheets and exercises, the book helps readers build a unique life plan that is about more than simply retirement. The book includes: A proprietary approach to retirement planning that changes seamlessly when times and circumstances change A four component  methodology  - Where You Are; What You Want; What to Do; and Make it Happen - to ensure continuous feedback, accountability, and measurement of lifetime goals Retirement planning expertise from wealth management firm Laird Norton Tyee    Wealth Regeneration at Retirement: Planning for a Lifetime of Leadership is artfully illustrated and filled with practical advice for wealthy baby boomers and the financial advisors they rely on.    It explains exactly how to build a personalized and sustainable plan for retirement no matter where life may lead.

  • Providing a comprehensive survey of the clinical, educational, developmental, psychosocial, and transitional issues relevant to people with Down syndrome, this book addresses the needs of family members, caregivers, and professionals alike. Edited in association with the National Down Syndrome Society, this up-to-date treatment incorporates the newest developments concerning sexuality, inclusion, transition into adulthood, and legislation, as well as a discussion of the Human Genome Project and the sequencing of chromosome 21.

  • From the reviews of the 3rd Edition... "The standard reference for anyone interested in understanding flow cytometry technology."
    American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to a wide audience?written in such an attractive way, being both informative and stimulating."
    Trends in Cell Biology This reference explains the science and discusses the vast biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. Now in its fourth edition, this text has been expanded to provide full coverage of the broad spectrum of applications in molecular biology and biotechnology today. New to this edition are chapters on automated analysis of array technologies, compensation, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, along with fully updated and revised references and a list of suppliers.

  • Principles of Psychopharmacology for Mental Health Professionals addresses the current "split" model of mental health care, in which physicians provide pharmaco-therapy while non-prescribing practitioners oversee other services such as psychotherapy. Bringing these two areas together, this book familiarizes mental health professionals with the medications used to treat psychiatric disorders.
    Prepared by world-renowned psychopharmacologists and psychiatrists, this useful resource helps non-prescribing practitioners understand when and why a given medication is appropriate to use, when it is not indicated, and what potential side effects may occur. Also discussed are the appropriate times in therapy when a referral for a medication evaluation is indicated as well as how to broach this issue with the patient. Recognizing that there are almost always a number of medications from which to choose, the authors combine research outcomes with their extensive clinical experience to highlight the important considerations in selecting one medication over another. As an educational tool, the text encourages and supports clinicians who wish to increase patients' understanding of treatment.
    After an introduction and overview, chapters cover:
    * Basics of psychopharmacology
    * Attention-deficit hyperactivity disorder
    * Mood disorders
    * Sleep disorders
    * Schizophrenia
    * Cognitive disorders
    * Anxiety disorders
    * Personality disorders
    * Substance use disorders
    * Traumatic brain injury
    * Eating disorders
    * Side effects
    Every chapter includes diagnostic considerations, history of medication treatments, and emerging trends for each disease.
    Principles of Psychopharmacology for Mental Health Professionals lays out what psychotherapists and other mental health practitioners need to know about psychotropic medications, giving them and their patients an invaluable guide to the full array of treatments available.

  • This newly updated edition of the benchmark guide to computer-assisted clinical trials provides a comprehensive primer for prospective managers. It covers every critical issue of the design and conduct of clinical trials, including study design, organization, regulatory agency liaison, data collection and analysis, as well as recruitment, software, monitoring, and reporting.
    Keeping the same user-friendly format as the original, this Second Edition features new examples and the latest developments in regulatory guidelines, such as e-submission procedures and computerized direct data acquisition. The new edition also reflects the increasing globalization of clinical trial activities, and includes new information about international standards and procedures, including the Common Technical Document and CDISC standards.
    This step-by-step guide is supported by handy checklists and extracts from submitted protocols. Experienced author and consultant Phillip Good incorporateshumorous yet instructive anecdotes to illustrate common pitfalls. Based on the proven industrial formula of planning, implementing, and finally performing essential checks, the book's three sections-"Plan," "Do," and "Check"-includethe following material:
    * Should the trials be conducted?
    * Put it in the computer and keep it there
    * Staffing for success
    * Designing trials and determining sample size
    * Budgeting
    * Recruiting and retaining patients and physicians
    * Data management
    * Monitoring the trials
    * Data analysis
    * After action review
    * Exception handling
    Executive and managerial professionals involved in the design and analysis of clinical experiments, along with clinical research associates, biostatisticians, and students in public health will find A Manager's Guide an indispensable resource.
    Praise for the First Edition:
    ". . . readable, informative and at times witty . . . never stops being concise and well written . . . a book worth a read . . ."
    -Statistics in Medicine
    "The book is very prescriptive and full of lists and tables with which to guide managers in making effective decisions in using computer-assisted clinical trials in pharmaceutical studies." -Technometrics
    "This book is must-have reading for anyone in the business . . ."
    -Clinical Chemistry

  • Functional Metabolism of Cells is the first comprehensive survey of metabolism, offering an in-depth examination of metabolism and regulation of carbohydrates, lipids, and amino acids. It provides a basic background on metabolic regulation and adaptation as well as the chemical logic of metabolism, and covers the interrelationship of metabolism to life processes of the whole organism. The book lays out a structured approach to the metabolic basis of disease, including discussion of the normal pathways of metabolism, altered pathways leading to disease, and use of molecular genetics in diagnosis and treatment of disease. It also takes a unique comparative approach in which human metabolism is a reference for metabolism in microorganisms and plant design, and presents novel coverage of development and aging, and human health and animal adaptation. The final chapter reviews the past and future promise of new genetic approaches to treatment and bioinformatics.
    This, the most exhaustive treatment of metabolism currently available, is a useful text for advanced undergraduates and graduates in biochemistry, cell/molecular biology, and biomedicine, as well as biochemistry instructors and investigators in related fields.

  • An Introduction to Human Molecular Genetics
    Second Edition
    Jack J. Pasternak
    The Second Edition of this internationally acclaimed text expands its coverage of the molecular genetics of inherited human diseases with the latest research findings and discoveries. Using a unique, systems-based approach, the text offers readers a thorough explanation of the gene discovery process and how defective genes are linked to inherited disease states in major organ and tissue systems. All the latest developments in functional genomics, proteomics, and microarray technology have been thoroughly incorporated into the text.
    The first part of the text introduces readers to the fundamentals of cytogenetics and Mendelian genetics. Next, techniques and strategies for gene manipulation, mapping, and isolation are examined. Readers will particularly appreciate the text's exceptionally thorough and clear explanation of genetic mapping. The final part features unique coverage of the molecular genetics of distinct biological systems, covering muscle, neurological, eye, cancer, and mitochondrial disorders. Throughout the text, helpful figures and diagrams illustrate and clarify complex material.
    Readers familiar with the first edition will recognize the text's same lucid and engaging style, and will find a wealth of new and expanded material that brings them fully up to date with a current understanding of the field, including:
    * New chapters on complex genetic disorders, genomic imprinting, and human population genetics
    * Expanded and fully revised section on clinical genetics, covering diagnostic testing, molecular screening, and various treatments
    This text is targeted at upper-level undergraduate students, graduate students, and medical students. It is also an excellent reference for researchers and physicians who need a clinically relevant reference for the molecular genetics of inherited human diseases.

  • Comparative Vertebrate Neuroanatomy
    Evolution and Adaptation
    Second Edition
    Ann B. Butler and William Hodos
    The Second Edition of this landmark text presents a broad survey of comparative vertebrate neuroanatomy at the introductory level, representing a unique contribution to the field of evolutionary neurobiology. It has been extensively revised and updated, with substantially improved figures and diagrams that are used generously throughout the text. Through analysis of the variation in brain structure and function between major groups of vertebrates, readers can gain insight into the evolutionary history of the nervous system. The text is divided into three sections:
    * Introduction to evolution and variation, including a survey of cell structure, embryological development, and anatomical organization of the central nervous system; phylogeny and diversity of brain structures; and an overview of various theories of brain evolution
    * Systematic, comprehensive survey of comparative neuroanatomy across all major groups of vertebrates
    * Overview of vertebrate brain evolution, which integrates the complete text, highlights diversity and common themes, broadens perspective by a comparison with brain structure and evolution of invertebrate brains, and considers recent data and theories of the evolutionary origin of the brain in the earliest vertebrates, including a recently proposed model of the origin of the brain in the earliest vertebrates that has received strong support from newly discovered fossil evidence
    Ample material drawn from the latest research has been integrated into the text and highlighted in special feature boxes, including recent views on homology, cranial nerve organization and evolution, the relatively large and elaborate brains of birds in correlation with their complex cognitive abilities, and the current debate on forebrain evolution across reptiles, birds, and mammals.
    Comparative Vertebrate Neuroanatomy is geared to upper-level undergraduate and graduate students in neuroanatomy, but anyone interested in the anatomy of the nervous system and how it corresponds to the way that animals function in the world will find this text fascinating.

  • Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. Written by leading experts in the field, Culture of Cells for Tissue Engineering offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized-part one discusses cell sourcing, preparation, and characterization and the second part examines specific engineered tissues Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies End-of-chapter features include useful listings of sources for reagents, materials, and supplies, with the contact details of the suppliers listed at the end of the book A section of elegant color plates to back up the figures in the chapters Culture of Cells for Tissue Engineering gives novice and seasoned researchers in tissue engineering an invaluable resource. In addition, the text is suitable for professionals in related research, particularly in those areas where cell and tissue culture is a new or emerging tool.

  • This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.

  • This comprehensive reference covers the comparative methodology involved in studying molecular evolution. Providing a practical introduction to the role of bioinformatics in comparative genomics, this publication further discusses the basic technology used in genome sequencing projects and provides an overview of genome storage databases currently in use.
    This timely and cutting-edge text also:
    * Reviews the basic principles of genomics and gene
    expression analysis
    * Discusses analytic methods in proteomics and
    * Includes a comprehensive list of Web resource

  • The Fourth Edition of Microbial Physiology retains the logical, easy-to-follow organization of the previous editions. An introduction to cell structure and synthesis of cell components is provided, followed by detailed discussions of genetics, metabolism, growth, and regulation for anyone wishing to understand the mechanisms underlying cell survival and growth. This comprehensive reference approaches the subject from a modern molecular genetic perspective, incorporating new insights gained from various genome projects.

  • This comprehensive publication covers all aspects of image formation in modern medical imaging modalities, from radiography, fluoroscopy, and computed tomography, to magnetic resonance imaging and ultrasound.  It addresses the techniques and instrumentation used in the rapidly changing field of medical imaging.  Now in its fourth edition, this text provides the reader with the tools necessary to be comfortable with the physical principles, equipment, and procedures used in diagnostic imaging, as well as appreciate the capabilities and limitations of the technologies.

  • Through analyses of the complex underlying issues, this interdisciplinary volume frames the agenda for dealing with genetic variation and incorporating pharmacogenomics into health care. The three sections of this book, Research Issues, Clinical Issues, and Social Perspectives  address key elements integral to a comprehensive discussion of this emerging field. This groundbreaking text... Examines new research strategies, methodologies, and ethical and social considerations of pharmacogenomics Addresses practical considerations of anticipated changes in education, training, oversight, guidelines and protocols, and continuing education requirements Provides analyses of the potential enormous impact of pharmacogenomics, such as in the standard of care and treatment, including perspectives from the fields of anthropology, law, ethics, and economics

  • A must-have far-reaching text that provides readers with a state-of-the-art molecule update on transmembrane transporters, focusing on the methodological approaches currently employed to better understand how transporters work and how they can be used in cutting edge therapies. Each chapter begins with an overview of the importnat biological questions presently being considered in their field, then presents scientific approaches to address these questions. In explaining approaches, the authors cover bench-top protocols, conceptual frameworks, data obtained, and pitfalls common to the techniques.

  • Wiley is proud to announce the publication of the first ever broad-based textbook introduction to Bioinformatics and Functional Genomics by a trained biologist, experienced researcher, and award-winning instructor. In this new text, author Jonathan Pevsner, winner of the 2001 Johns Hopkins University "Teacher of the Year" award, explains problem-solving using bioinformatic approaches using real examples such as breast cancer, HIV-1, and retinal-binding protein throughout. His book includes 375 figures and over 170 tables. Each chapter includes: Problems, discussion of Pitfalls, Boxes explaining key techniques and math/stats principles, Summary, Recommended Reading list, and URLs for freely available software. The text is suitable for professionals and students at every level, including those with little to no background in computer science.

  • Written for biologists and medical researchers who don't have any special training in data analysis and statistics, Guide to Analysis of DNA Microarray Data, Second Edition begins where DNA array equipment leaves off: the image produced by the microarray. The text deals with the questions that arise starting at this point, providing an introduction to microarray technology, then moving on to image analysis, data analysis, cluster analysis, and beyond.
    With all chapters rewritten, updated, and expanded to include the latest generation of technology and methods, Guide to Analysis of DNA Microarray Data, Second Edition offers practitioners reliable information using concrete examples and a clear, comprehensible style. This Second Edition features entirely new chapters on:
    * Image analysis
    * Experiment design
    /> * Automated analysis, integrated analysis, and systems biology
    * Interpretation of results
    Intended for readers seeking practical applications, this text covers a broad spectrum of proven approaches in this rapidly growing technology. Additional features include further reading suggestions for each chapter, as well as a thorough review of available analysis software.

  • Microarray technology provides researchers in the life sciences with a revolutionary tool for measuring gene expression. However, this highly developed process involves multiple steps, from sample selection to data analysis, each susceptible to potentially costly errors. Without sound quality control, experimental microarrays may produce useless or, even worse, misleading results.
    Microarray Quality Control provides a comprehensive resource for ensuring quality control in every step of this complex process. From experimental design to data processing, analysis, and interpretation, the emphasis in this text remains on practical advice for each stage of planning and running a microarray study. Chapters cover:
    * Quality of biological samples
    * Quality of DNA
    * Hybridization protocols Scanning
    * Data acquisition
    * Image analysis
    * Data analysis
    /> Written for the broad group of workers-biologists, mathematicians, statisticians, engineers, physicians, and computational scientists-involved in microarray studies, Microarray Quality Control features a straightforward style easily accessed by various disciplines. Useful checklists and tips help ensure the integrity of results, and each chapter contains a thorough review of pertinent literature.
    The only complete, systematic treatment of the topic available, Microarray Quality Control offers students and practitioners an invaluable resource for improving experimental quality and efficiency.